ImageInLife - Imaging the Vertebrate embryo: from biological material to modelling
Ref. 08019
Learn how to image developmental & biological processes at cellular or subcellular resolution in a complete organism.
- Duration: 5 weeks
- Effort: 10 hours
- Pace: ~2 hours/week
- Languages: English
What you will learn
At the end of this course, you will be able to:
- How to get vertebrate embryos to be imaged
- How to label embryos and larvae for imaging
- How to choose the right microscope for your purpose
- How to deal with both the type of data generated and with the large amount of images/data generated by your microscope
- How to choose the right modeling tools and what modelling could bring to the experimental plans and to the understanding of the underlying biological processes.
Description
Microscopy technologies have progressed to such a point that it is now possible to image a developing vertebrate at the single cell resolution. Two main model species are being used for such studies, the zebrafish and the mouse.
The present MOOC introduces the trainees to these two animal models, to the most modern microscopy technologies, to the use of high volume of data (images) and to the modelling of the studied processes. Trainees will also be introduced to the intravital study of viral infections.
The MOOC gives both a general introduction and introduces to more specialized tools and concepts.
Format
The MOOC is made of 5 weeks of electronic lessons, and you will be offered quizzes to evaluate your performances
Prerequisites
The target audience are :
- Students in their first years at the University, before graduating a Bachelor, in order for them to be introduced to the challenges of intravital imaging in vertebrates before their first laboratory internship.
- Master students interested in these disciplines or graduated students and researchers that want to implement research projects in these disciplines.
Prerequisite : Basic knowledge in biology
Assessment and certification
At the end of each week, quizzes are available to evaluate your acquired knowledge.
Course plan
- Week 1 : Preparing embryos for imaging
- Week 2 : What to image; Labelling techniques
- Week 3 : How to image, Microscopes
- Week 4 : From images to data
- Week 5 : Modelling
Course team
Tamara Sipka
Categories
from Serbia, Marie Skłodowska-Curie PhD student at the University of Montpellier, France
Salomé Muñoz
Categories
from Chile, Marie Skłodowska-Curie PhD student at Leiden University, The Netherlands
Radoslaw Gora
Categories
from Poland, Marie Skłodowska-Curie PhD student at Leiden University, The Netherlands
Matteo Bernardello
Categories
from Italy, Marie Skłodowska-Curie PhD student at ICFO, Barcelona, Spain
Joel Dokmegang
Categories
from Cameroon, Marie Skłodowska-Curie PhD student at Manchester Metropolitan University, UK
Laurent Thomas
Categories
from France, Marie Skłodowska-Curie PhD student at Acquifer, Heidelberg, Germany
Polycarp Okock
Categories
from Kenya, Marie Skłodowska-Curie PhD student at TatraMed, Bratislava, Slovakia
Valerio Laghi
Categories
from Italy, Marie Skłodowska-Curie PhD student at Institut Pasteur, Paris, France
Organizations
Partners
License
License for the course content
Attribution-ShareAlike
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
License for the content created by course participants
Attribution-ShareAlike
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.